Quantitative Estimation of Agrobacterium tumefaciens DNA in Crown Gall Tumor Cells
نویسندگان
چکیده
منابع مشابه
Plant responses to Agrobacterium tumefaciens and crown gall development
Agrobacterium tumefaciens causes crown gall disease on various plant species by introducing its T-DNA into the genome. Therefore, Agrobacterium has been extensively studied both as a pathogen and an important biotechnological tool. The infection process involves the transfer of T-DNA and virulence proteins into the plant cell. At that time the gene expression patterns of host plants differ depe...
متن کاملIsolation of Agrobacterium Tumefaciens Strains from Crown Gall Disease on Imported Roses Plants in Qazvin Province
Cultivar of roses (Rosa spp.) has been grown in greenhouses in Qazvin region of Iran for local markets. Agrobacterium tumefaciens strains were isolated and identified from six different samples of roses plants imported from the Netherland to Iran. During August and September of 2012, nearly 2-5% of rose plants in two different greenhouses in the province of Qazvin were observed with crown gall ...
متن کاملAgrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.
Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 10(4)-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per ...
متن کاملMechanisms of tumor induction in crown gall. I. Production and pathogenicity of spheroplasts of Agrobacterium tumefaciens.
The hypothesis that crown gall tumorigenesis requires the formation of spheroplasts was tested by comparing the tumorinducing ability of populations of both permanent and revertable spheroplasts of Agrobacterium tumefaciens with that of popula tions of the typical rod form of the bacterium. Permanent spheroplasts produced by serial transfers in medium containing 0.005 MD-methionine did not prod...
متن کاملAgrobacterium tumefaciens Site Attachment as a Necessary Prerequisite for Crown Gall Tumor Formation on Potato Discs.
The infectivity of Agrobacterium tumefaciens strain B6 was inhibited about 50% when these bacteria were inoculated on potato discs with equal viable cell counts of a weakly virulent strain of A. tumefaciens (B-48) or autoclaved strains of B6 or B-48. Inhibition by B-48 or autoclaved B6 could still be obtained when these cells were added up to a maximum of 10 minutes after the addition of viable...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1974
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.71.9.3677